Appeal No. 1995-2297
Application No. 07/797,493
a. sublethally irradiating an immunodeficient mouse, wherein the
immunodeficient mouse lacks population of functional T cells and B cells;
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b. infusing from about 10 to about 10 human hematopoietic cells into
the immunodeficient mouse;
c. administering an amount of MGF and GM-CSF/IL-3 FP which is
effective to promote engraftment of the human hematopoietic cells, and an amount of an
additional, lineage-specific human growth factor, selected from the group consisting of
erythropoietin (EPO), granulocyte-colony stimulating factor (G-CSF), granulocyte
macrophage-colony stimulating factor (GM-CSF), interleukins-2, -4, -5, -6, and -7, and
combination thereof which is effective to promote differentiation and proliferation of the
desired lineages of human hematopoietic cells.
The references relied upon by the examiner are:
European Patent Application
Reisner 0 438 053 Jul. 24, 1991
Fohlmeister et al. (Fohlmeister), “The Possibility of Assaying Wistar Rat Bone Marrow
CFUs in a Xenogeneic (Rat-to-Mouse)System” 4 Cell Growth Regul. pp 221-28 (1985)
Pollard (Pollard), “Protected Environment and its Utility in Experimental Allogeneic and
Xenogeneic Bone Marrow Transplantation” 10 (No. 2,3) Tokai J. Exp. Clin Med., pp. 175-
79 (1985)
Wade et al. (Wade), “Characterization of Xenogeneic Mouse-To-Rat Bone Marrow
Chimeras” 44 (No. 1) Transplantation pp. 88-92 (1987)
McCune, et al. (McCune), “The SCID-hu Mouse: Murine Model for the Analysis of Human
Hematolymphoid Differentiation and Function” 241 Science pp. 1632-39 (1988)
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