Appeal No. 1997-3322
Application No. 08/353,940
Claims 1 and 31 are illustrative of the claims on appeal and read as follow:
1. A cloning vector which expresses and secretes V or V T-cell receptor variable " $
domain in a gram-negative cell, said secretion being into the bacterial periplasm or into a
culture medium, said vector comprising the following elements in the 5' to 3' direction, said
elements which are operatively linked:
(a) an inducible promoter DNA sequence;
(b) a leader sequence; and
(c) a DNA sequence encoding a V or V T-cell receptor variable domain." $
31. A method for expressing and secreting a T-cell receptor variable domain in
a gram-negative bacterium, comprising the steps:
a) culturing a gram-negative bacterium transformed with a vector, said
vector comprising the following elements in the 5' to 3' direction, said
elements which are operatively linked:
i) a lacZ inducible promoter DNA sequence;
ii) a leader sequence selected from the group consisting of pelB,
ompA and phoA; and
iii) a DNA sequence encoding at least one of a V or V T-cell receptor " $
variable domain; and
b) adding about 0.1 to 1 mM of isopropylthiogalactopyranoside;
to produce a T-cell receptor variable domain.
The prior art references of record relied upon by the examiner in rejecting the
appealed claims are:
Novotny et al. (Novotny 1986), “Secondary, tertiary, and quaternary structure of T-cell-
specific immunoglobulin-like polypeptide chains,” Proc. Natl. Acad. Sci. USA, Vol. 83, pp.
742-46 (1986).
Skerra et al. (Skerra), “Assembly of a Functional Immunoglobulin F Fragment in
v
Escherichia coli,” Science, Vol. 245, pp. 1040-41 (1988).
Novotny et al. (Novotny 1991), “Assoluble, dingle-chain T-cell receptor fragment endowed
with antigen-combining properties,” Proc. Natl. Acad. Sci USA, Vol. 88,
pp. 8646-650 (1991).
2
Page: Previous 1 2 3 4 5 6 7 8 9 10 11 12 13 Next
Last modified: November 3, 2007