Appeal No. 2001-0246
Application No. 08/921,103
Representative independent claim 47 is reproduced as
follows:
47. A method of providing reference signal in a system for
potentiometric quantitative analysis, said method comprising the
steps of:
(a) providing a flow cell in any of a plurality of
electrode receiving positions on a sensor support member of said
system, each of said electrode receiving positions adapted to
support one of a plurality of working electrodes or said flow
cell, wherein said flow cell is adapted to be serially retained
within a sample flow path of the system;
(b) interfitting said flow cell in series with ones of
said plurality of working electrodes within said plurality of
electrode receiving positions;
© storing a liquid junction solution in a remote
reservoir connected to said flow cell for delivery of said
liquid junction solution to said flow cell and for receipt of
said liquid junction solution therefrom;
(d) moving a sample solution through said sample flow path
to said flow cell to form a liquid junction between the sample
solution and said liquid junction between the sample solution
and said liquid junction solution about a porous constraint
whereby said sample solution is disposed in contact with one
side of said porous constraint, said constraint being adapted to
substantially prevent bulk flow of said liquid junction solution
therethrough; and
(e) circulating said liquid junction solution, comprising
a non-saturated equitransferent salt, in conjunction with said
step of moving said sample solution, whereby a volume of said
liquid junction solution is pumped from said remote reservoir
towards said flow cell and from said flow cell towards said
reservoir, whereby said liquid junction solution is disposed in
contact with an other side of said porous constraint and whereby
said liquid junction solution is electrically connectable to
sensing equipment by a reference contact region in physical
contact with said liquid junction solution, said reference
contact region providing said reference.
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