Ex Parte CHAN et al - Page 7




          Appeal No.  2001-0246                                                       
          Application No.  08/921,103                                                 

          reference fluid enters the bridge through inlet tube 15 directly            
          or indirectly from a container and exits through outlet tube 16             
          directly or indirectly to a suitable reservoir (col. 2, lines               
          49-55).  In fact, Europe teaches that the reference fluid flows             
          to and from a container into chamber 21 on one side of the                  
          porous bridge element while the sample fluid flows on the other             
          side of the bridge for chemistry analyzing.                                 
               Hofmeier, on the other hand, relates to a free-diffusion               
          liquid junction in which the reference fluid is pumped from                 
          reservoir 17 and returned to the reservoir after the reading of             
          the sample fluid.  As shown in Figure 1, Hofmeier discloses that            
          sample fluid 2 is purged from measuring channel 3 by cleansing              
          fluid 9 after each reading is completed (col. 4, lines 16-22).              
          However, Hofmeier returns reference fluid 18 to the same                    
          container after the reading (col. 4, lines 26-32) and reuses the            
          reference fluid for future readings.  Similarly, Covington                  
          discloses that the flow rate of the reference fluid in a fresh              
          liquid junction (free junction) is set such that a fresh liquid             
          junction may be established each time the sample fluid is                   
          removed.  Covington further suggests that the pumps need only be            
          running long enough to reform or refresh the junction by flowing            
          a small amount of the reference fluid (page 224).                           

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