Appeal No. 2002-2030 Page 15
Application No. 09/294,663
Instant claims 6 and 9 compare to the ‘558 patent’s claims 7 and 8 as
follows:
Instant claim: ‘558 patent claim:
6. A method of producing an IIM 7. A method of producing an
protein or peptide comprising: invertebrate intestinal mucin protein or
peptide comprising:
a) transforming a host cell with
an expression vector comprising a a) transforming a host cell with an
promoter operatively linked to a expression vector comprising a
nucleotide sequence which codes for a promoter operatively linked to a
predetermined protein or peptide of an nucleotide sequence, wherein the
IIM protein; nucleotide sequence encodes an
amino acid sequence selected from the
b) culturing said host cell under group consisting of:
conditions such that said IIM protein is i) SEQ ID No. 3; and
expressed in recoverable quantity; ii) SEQ ID No. 4;
or wherein the nucleotide
c) lysing said host cell; and sequence encodes a peptide
comprised by a sequence selected
d) recovering said IIM protein. from the group consisting of:
i) SEQ ID No. 3; and
ii) SEQ ID No. 4;
b) culturing said host cell under
conditions that allow expression of said
invertebrate intestinal mucin protein or
peptide in recoverable quantity;
c) lysing said host cell; and
d) recovering said invertebrate
intestinal mucin protein or peptide.
9. The method of claim 6 wherein said 8. The method of claim 7 wherein said
expression vector further comprises a expression vector encodes a fusion
gene encoding a transfer molecule protein comprising the invertebrate
such as glutathione-S-transferase. intestinal mucin protein or peptide and
glutathione-S-transferase.
Instant claims 21 and 22 are the same as instant claims 6 and 9 except
that claims 21 and 22 are limited to a method of making a “Trichoplusia ni IIM
protein,” rather than any “IIM protein or peptide.”
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