Appeal No. 2004-1967 Page 3
Application No. 09/027,439
target nucleic acid sequences . . . [wherein] a target nucleic acid sequence contains a
species-specific or genus specific nucleotide.” Specification, page 17. Targeting
probes are “complementary to areas of rRNA or rDNA having species-specific
nucleotides or genus-specific nucleotides” and are capable of “detecting and
discriminating . . . the presence of rRNA or rDNA molecules of Shigella species and E.
coli.” Id., page 13. Claims 55 and 56 are representative (differences underlined):
55. A probe which
a) targets Shigella flexneri comprising a fragment greater than 10 to 40 bases in
length of a nucleotide sequence SEQ ID NO: 3, an RNA equivalent thereof, or a nucleic
acid complementary to said molecule, capable of base-pairing according to the standard
Watson-Crick complementarity rules,
b) targets Shigella sonnei comprising a fragment greater than 10 to 40 bases in
length of a nucleotide sequence SEQ ID NO: 4, an RNA equivalent thereof, or a nucleic
acid complementary to said molecule, capable of base-pairing according to the standard
Watson-Crick complementarity rules,
c) targets Shigella dysenteriae comprising a fragment greater than 10 to 40
bases in length of a nucleotide sequence SEQ ID NO: 5, an RNA equivalent thereof, or
a nucleic acid complementary to said molecule, capable of base-pairing according to
the standard Watson-Crick complementarity rules, or
d) targets Shigella boydii comprising a fragment greater than 10 to 40 bases in
length of a nucleotide sequence SEQ ID NO: 6, an RNA equivalent thereof, or a nucleic
acid complementary to said molecule, capable of base-pairing according to the standard
Watson-Crick complementarity rules.
56. A probe which
a) targets Shigella flexneri consisting of a fragment greater than 10 to 40 bases
in length of a nucleotide sequence SEQ ID NO: 3, an RNA equivalent thereof, or a
nucleic acid complementary to said molecule, capable of base-pairing according to the
standard Watson-Crick complementarity rules,
b) targets Shigella sonnei consisting of a fragment greater than 10 to 40 bases in
length of a nucleotide sequence SEQ ID NO: 4, an RNA equivalent thereof, or a nucleic
acid complementary to said molecule, capable of base-pairing according to the standard
Watson-Crick complementarity rules,
c) targets Shigella dysenteriae consisting of a fragment greater than 10 to 40
bases in length of a nucleotide sequence SEQ ID NO: 5, an RNA equivalent thereof, or
a nucleic acid complementary to said molecule, capable of base-pairing according to
the standard Watson-Crick complementarity rules, or
d) targets Shigella boydii consisting of a fragment greater than 10 to 40 bases in
length of a nucleotide sequence SEQ ID NO: 6, an RNA equivalent thereof, or a nucleic
acid complementary to said molecule, capable of base-pairing according to the standard
Watson-Crick complementarity rules.
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