Appeal No. 2004-1967 Page 5
Application No. 09/027,439
hybridization at 40°-65°C for 14-16 hours in a hybridization solution at pH 7.8,
containing 0.9 M NaCl, 0.12 M Tris-HCl, 6 nM EDTA, 0.1 M sodium phosphate buffer,
0.1% SDS and 0.1% polyvinylpyrrolidone,
followed by three 15-minute washes at 40°-65°C to remove unbound probes in a
solution at pH 7, containing 0.075 M NaCl, 0.0075 M Na Citrate and 0.1% SDS.
53. An isolated nucleic acid molecule comprising a nucleotide sequence of SEQ
ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6,
or an RNA equivalent thereof,
or a nucleic acid complementary to said isolated molecule, capable of base-
pairing according to standard Watson-Crick complementarity rules.
The claims stand rejected as follows:
I. Claims 55-58 under 35 U.S.C. § 112, second paragraph, as indefinite.
II. Claims 55-58 under 35 U.S.C. § 101 as “directed to non-statutory subject
matter.
III. Claims 47, 48, 53 and 55-58 under 35 U.S.C. § 102(a) and (e) as anticipated
by Hogan.2
IV. Claims 47, 48, 53 and 55-58 under 35 U.S.C. § 102(a) as anticipated by
Genbank Accession No. X969643 or X807264 (disclosed in Cilia5).
V. Claims 47, 48, 53 and 55-58 under 35 U.S.C. § 103 as unpatentable over
Genbank Accession No. A145656 and Dyson.7
2 Hogan et al., U.S. Patent 5,541,308, issued July 30, 1996.
3 Genbank Accession No. X96964, February 4, 1996.
4 Genbank Accession No. X80726, March 29, 1996.
5 Cilia et al., “Sequence Heterogeneities Among 16S Ribosomal RNA
Sequences, and Their Effect on Phylogenetic Analyses at the Species Level,” Mol. Biol.
Evol., Vol. 13, No. 3, pp. 451-461 (February 20, 1996).
6 Genbank Accession No. A14565, September 29, 1994.
7 Dyson, N.J., in Essential Molecular Biology Vol. II: A Practical Approach,
Chapter 5, pp. 11-156, Brown, T.A. ed., Oxford University Press, 1992.
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