Appeal No. 2005-0543 Page 2
Application No. 09/992,221
(b) staining the sample with a fluorescent dye for producing
a difference in intensity of fluorescence among the leukocytic cells,
the erythroid cells, and the lipid particles;
(2) introducing the resulting sample to a flow cytometer to detect at
least one kind of scattered light and at least one kind of
fluorescence;
(3) classifying the lipid particles, the leukocytic cells and the
erythroid cells by the difference in the intensities of their
fluorescence and their scattered light; and
(4) obtaining a count of the leukocytic cells and erythroid cells in the
step of (3).
11. A method of classifying and counting leukocytic cells and erythroid
cells in a bone marrow fluid comprising leukocytic cells and
erythroid cells and lipid particles comprising the steps of:
(1) (a) mixing a sample of the bone marrow fluid with an
erythrocyte lysing agent to lyse erythrocytes in the sample, thereby
rendering leukocytic cells, erythroid cells and lipid particles in the
sample suitable for staining, and
(b) staining the sample with a fluorescent dye for producing
a difference in intensity of fluorescence among the leukocytic cells,
the erythroid cells, and the lipid particles;
(2) introducing the resulting sample to a flow cytometer to detect
side scattered light and at least one kind of fluorescence;
(3) classifying the lipid particles, the leukocytic cells and the
erythroid cells by the difference in the intensities of their
fluorescence and their scattered light; and
(4) obtaining a count of the leukocytic cells and erythroid cells in the
step of (3).
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