Appeal No. 2006-0103 Page 3
Application No. 10/172,892
17 as representative; claims 2-9 and 11-16 will stand or fall with claim 1. Claims 1, 10,
and 17 reads as follows:
1. A method of performing an array hybridization experiment comprising:
contacting a target solution with an array disposed in an assay
chamber, wherein the assay chamber is substantially defined by a
substrate, a cover, and a form-in-place gasket, the form-in-place gasket
positioned between the substrate and the cover, wherein the contacting is
done under conditions and for a period of time sufficient to allow specific
binding interactions between the target solution and the array, and
Interrogating the array.
10. The method of claim 1, wherein contacting comprises depositing the target
solution into a well defined at least in part by one of the substrate or the
cover and placing the other one of the substrate or cover over the well with
the form-in-place gasket positioned between the substrate and the cover
such that a fluid tight seal is formed by the form-in-place gasket positioned
between the substrate and the cover.
17. A method of performing an array hybridization experiment comprising:
contacting a target solution with an array disposed in an assay
chamber, wherein the contacting is done under conditions and for a period
of time sufficient to allow specific binding interactions between the target
solution and the array, wherein the assay chamber is substantially defined
by a substrate, a cover, and a form-in-place gasket, the form-in-place
gasket positioned between the substrate and the cover, wherein the form-
in-place gasket comprises a gasket formed by a process comprising
depositing a fluid gasket material on a one or more of the substrate and the
cover and curing the fluid gasket material,
the method of performing an array hybridization experiment further
comprising:
interrogating the array.
Thus, claim 1 is directed to a method of performing an array hybridization
experiment using an assay chamber comprising a substrate and a cover, separated by a
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