Appeal 2006-0592
Application 10/278,274
DISCUSSION
Claims 1 and 15, the only independent claims on appeal, are
representative of the claimed subject matter:
1. A method of producing an array of at least two different nucleic
acid ligands covalently bonded to a surface of a substrate, said method
comprising:
(a) providing a substrate having a surface displaying functional
groups;
(b) contacting blocked nucleoside monomers to at least a first
location and a second location of said surface under conditions sufficient for
said blocked nucleoside monomers to covalently bond to said surface in said
first and second locations to produce a substrate surface displaying
covalently bound blocked monomers, wherein said blocked nucleoside
monomers are blocked with a labile blocking group;
(c) contacting said surface displaying blocked nucleoside
monomers with an oxidation solution to produce an oxidized surface;
(d) washing said oxidized surface with an organic solvent having a
vapor pressure at standard temperature and pressure (STP) of less than about
13 KPa;
(e) contacting said oxidized surface with a deblocking solution that
comprises a deblocking agent in said organic solvent to produce a deblocked
surface;
(f) removing excess deblocking solution from said deblocked
surface; and
(g) reiterating steps (b) to (f) least once to produce said array of at
least two different nucleic acid ligands.
15. A method of producing an array of at least two different nucleic
acid ligands covalently bonded to a surface of a substrate, said method
comprising:
(a) providing a substrate having a surface displaying hydroxyl
functional groups;
(b) contacting DMT blocked nucleoside monomers via pulse-jet
deposition to at least a first location and a second location of said surface
under conditions sufficient for said DMT blocked nucleoside monomers to
2
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Last modified: September 9, 2013