Appeal 2006-0592
Application 10/278,274
covalently bond to said surface in said first and second locations to produce
a substrate surface displaying covalently bound blocked monomers;
(c) immersing said substrate in a volume of an oxidation solution
to produce a substrate having an oxidized surface;
(d) washing said oxidized surface with toluene;
(e) immersing said substrate having an oxidized surface in a
volume of a deblocking solution that comprises an acid in toluene to produce
a substrate having a deblocked surface;
(f) removing excess deblocking solution from said substrate having
a deblocked surface by dripping; and
(g) reiterating steps (b) to (f) least once to produce said array of at
least two different nucleic acid ligands.
Claims 1 and 15 recite the same method steps, in the same order, but
differ in breadth. For example, both claims require a substrate displaying
functional groups in step (a), but claim 15 requires that the functional groups
are hydroxyl groups. Both claims require blocked nucleoside monomers in
step (b), but claim 15 requires DMT-blocked nucleoside monomers. Both
claims require that the organic solvent used to wash the oxidized substrate in
step (d) is the same organic solvent used in the deblocking solution of step
(e). However, claim 1 merely requires any organic solvent that has a vapor
pressure at STP of less than about 13 KPa, while claim 15 specifies that the
organic solvent is toluene. Claim 15 specifies that the blocking agent in the
blocking solution of step (e) is an acid. Finally, both claims require that
excess deblocking solution is removed before adding additional nucleoside
monomers to the substrate, but step (f) of claim 15 specifies that the
deblocking solution is removed by dripping.
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