Appeal No. 2007-0056 Page 2
Application No. 09/906,511
of the binding partner and a known amount of sensitized
particles in an assay medium to form a second reaction
mixture, wherein said sensitized particles comprise carrier
particles having immobilized thereon said analyte of
interest;
b) incubating said first or second reaction mixture under
conditions that allow the formation of an aggregation
reaction mixture comprising said sensitized particles and
different sized aggregates thereof formed as a result of an
interaction between said analyte and said binding partner;
c) determining a size distribution of said sensitized particles
and said different sized aggregates thereof contained in
said reaction mixture by polarization intensity differential
scattering; and
d) determining the concentration of said analyte in said
sample using the distribution of said sensitized particles
and said different sized aggregates.
3. The method of claim 1, wherein said determining includes measuring
said particle size distribution as a function of time, wherein the change
in the distribution of aggregate size as a function of time is utilized to
determine the amount of said analyte in said sample.
8. The method of claim 1, wherein said insoluble particles are selected
from the group consisting of polystyrene, acrylonitrile, polybutadiene,
acrylamide, methacrylate, nylon, metals, metal oxides and their
derivatives, glass, dextran, cellulose, red blood cells, pollens,
liposomes, and bacteria.
The references relied upon by the examiner are:
Bott et al. (‘978) 4,953,978 Sep. 4, 1990
Bott et al. (‘221) 4,104,221 Apr. 14, 1992
Kosako 5,527,714 Jun. 18, 1996
Chandler et al. (Chandler) 5,981,180 Nov. 9, 1999
Hansen WO 92/21024 Nov. 26, 1992
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