Appeal 2007-0725
Reexamination Control 90/006,785
Patent 5,073,484
22. A method for the analysis of an analyte which is a member of a
ligand-antiligand binding pair in a test solution comprising the steps
of:
(a) providing a non-diffusively immobilized reactant in each of one or
more reaction zones spaced successively along a flow path defined by
a liquid permeable medium, wherein said reactant is the other member
of said binding pair and is capable of binding with the analyte to form
a predetermined product;
(b) flowing said solution along the medium and sequentially through
the reaction zone(s); and
(c) detecting the presence of analyte, said reactant or said
predetermined product in the reaction zone(s), wherein the number of
zones in which detection occurs is related to the presence of analyte in
the solution.
23. The method of claim 22 wherein one of the members of the
ligand-antiligand binding pair is labeled with a chemical moiety, and
wherein said detecting step comprises detecting the presence of said
chemical moiety.
24. The method of claim 23 wherein the chemical moiety includes an
enzyme.
25. The method of claim 23 wherein the chemical moiety is a radioisotope.
2. Regarding the detecting step, the ‘484 patent states the following:
The detectors useful in the invention are capable of detecting
the presence in the successive reaction zones, of analyte, analyte
derivatives, reactants, or the predetermined reaction product, all as
described above. The means of detection may take various forms. In
the preferred embodiment, detection is signaled by a change of color,
or a lack of a change of color, in the respective reaction zones of the
apparatus. However, detection may be signaled by other means as
well, such as by luminescence or fluorescence of the zones,
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