Appeal 2007-1624
Application 10/424,662
arrays on said substrate correlates with the position of the
corresponding reservoir.
FINDINGS OF FACT
Fodor
1. Fodor describes positionally attaching oligonucleotides to a substrate to
produce “substrates of positionally definable sequence specific probes”
(Fodor, at col. 3, ll. 49-53).
2. “Usually the specific reagents are all attached to a single solid substrate,
and the reagents comprise about 3000 different sequences. . . . Usually, the
reagents are localized in regions of the substrate having a density of at least
25 regions per square centimeter” (Fodor, at col. 3, ll. 6-13).
3. Fodor also describes an embodiment in which the substrates are beads
(Fodor, at col. 3, l. 58).
4. Each bead can have a single oligonucleotide probe type (“reagent”)
attached to it (Fodor, at col. 4, ll. 1-4; at col. 21, ll. 42-43).
5. The beads may be “encoded to indicate the subsequence specificity of
[the reagent on the bead]” (Fodor, at col. 21, ll. 44-46).
6. “[T]he target [polynucleotide] may be bound to the whole collection of
beads and those beads that have appropriate specific reagents [e.g.,
oligonucleotides] on them will bind to the target. Then a sorting system may
be utilized to sort those beads that actually bind the target from those that do
not” (Fodor, at col. 21, ll. 48-54).
7. After the beads “which have bound the target have been collected, the
encoding scheme may be read off to determine the specificity of the reagent
on the bead. An encoding system may include a magnetic system, a shape
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