Appeal 2007-2955
Application 10/190,425
were then gently washed off . . . Arrays were then fixed” (Belov 4484,
paragraph bridging left- and right-hand col.).
29. According to Belov, “[t]he main strength of the [ ] Array is speed and
extensive immunophenotyping, enabling pattern recognition using large
arrays of microscopic antibody dots” (Belov 4488, left-hand col.).
Chang
30. Chang describes a device “compris[ing] a pattern or array of minute
antibody-coated spots on the surface of a support. Each antibody-coated
spot is made up of antibodies of a different and distinct specificity. The
spots serve as tiny, specific immuno-absorbents of cells” (Chang, col. 1, ll.
59-63).
31. “The density of antibodies in the antibody-coated spots is related to
the function of the spots as immunoadsorbents of cells” (Chang, col. 3, ll.
9-11). “The density required to generate a microscopically uniform layer of
cells is probably greater than the density minimally sufficient for tight cell
binding” (id. at col. 3, ll. 27-30). “The preferred concentration of antibodies
in the solution applied to the surface for coating is about 10 μg/ml” (id. at
col. 3, ll. 56-58).
32. “Preferably, the support is a solid substance . . . such as a glass or
plastic coverslip” and “[t]he antibodies in the antibody coated spots are
conjugated to the surface of the support . . . either covalently or non-
covalently” (Chang, col. 2, ll. 46-52).
33. “Cells to be tested for the presence of certain surface antigens are
placed in suspension in normal saline and applied to the surface of the
device” (Chang, col. 4, ll. 18-31).
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