Appeal 2007-3395
Application 10/260,733
(b) contacting further replicas of the array
with mixtures of the first nucleic acid sample and a
third nucleic acid sample and fractional dilutions
of the third sample, wherein the third sample
comprises a genomic nucleic acid sample with an
unknown karyotype and is labeled with the second
detectable label, and the genomic nucleic acid of
the third sample comprises a substantially
complete complement of genomic nucleic acid of a
third genome from a test cell or a tissue sample,
wherein the contacting is under conditions wherein
the nucleic acid in the mixtures of each of the first
and second samples and the first and third samples
can specifically hybridize to the genomic nucleic
acid segments immobilized on the array;
(c) measuring the amount of first label and
second label on each spot for each respective
contacted array and determining the karyotype of
each dilution fraction by comparative genomic
hybridization; and,
(d) selecting which fractional dilution
karyotype determination of the second sample
most closely determines the known karyotype, and
selecting data for the same fractional dilution of
the third sample to determine the karyotype of the
third sample, thereby determining the degree of
genetic mosaicism in the cell population.
Claim 34 limits the array-immobilized genome of the method of claim 1 to a
wild-type (normal) genome, while claim 35 limits the first sample of the
method of claim 34 to a wild-type (normal) genome. Claim 37 requires the
second sample of the method of claim 1 to have a mosaic karyotype. [Br. at
13.]
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