Appeal 2007-3395
Application 10/260,733
[10] The methods described in Appellant's specification are said to
determine "the number of karyotype subpopulations in a sample and
the percent of the cell population having a particular karyotype"
(Specification at 18, ¶ 55).
[11] In one embodiment, the specification describes a method comprising
(a) providing an array comprising a plurality of
cloned genomic nucleic acid segments, wherein
each genomic nucleic acid segment is immobilized
to a discrete and known spot on a substrate surface
to form an array and the cloned genomic nucleic
acid segments comprise a substantially complete
first genome of a known karyotype; (b) providing
a first sample, wherein the sample comprises a
plurality of genomic nucleic acid segments
comprising a substantially complete complement
of the first genome labeled with a first detectable
label; (c) providing a second sample, wherein the
sample comprises a plurality of genomic nucleic
acid labeled with a second detectable label, and the
genomic nucleic acid sample comprises a
substantially complete complement of genomic
nucleic acid of a cell or a tissue sample, and the
karyotype of the second sample is known and is
different from that of the first sample of step (b);
(d) providing a third sample, wherein the sample
comprises a genomic nucleic acid sample with an
unknown karyotype labeled with the second
detectable label, and the genomic nucleic acid
comprises a substantially complete complement of
genomic nucleic acid of a cell or a tissue sample;
(e) preparing serial dilution fractions of the
samples of steps (c) and (d); (f) contacting the
sample of step (b) separately with each serial
dilution fraction of the sample of step (c) with the
array of step (a) under conditions wherein the
nucleic acid in the samples can specifically
hybridize to the genomic nucleic acid segments
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