Appeal 2007-3395
Application 10/260,733
[21] Various control nucleic acid target elements may also be included on
the array, e.g. total genomic DNA or total genomic or cDNA from a
tissue with known abnormalities (Bao at col. 11, ll. 13-18).
[22] The first nucleic acid population comprises a mixture of mRNA or its
complementary cDNA, which is representative of gene expression in
the tissue sample and is labeled with a first marker, e.g., a red
fluorescent dye (Bao at col. 6, ll. 34-38; col. 17, ll. 5-9).
[23] The second nucleic acid population comprises a mixture of genomic
DNA which is representative of the tissue sample's total genomic, i.e.,
chromosomal, DNA and is labeled with a second marker, e.g., a green
fluorescent dye (Bao at col. 6, ll. 34-40; col. 17, ll. 5-9).
[24] The third nucleic acid population comprises a reference population
labeled with a third marker, e.g., an orange fluorescent dye (Bao at
col. 6, ll. 40-42; col. 17, ll. 5-9).
[25] The reference population may be total human genomic DNA from
normal tissue (Bao at col. 3, ll. 60-62; col. 6, ll. 46-49; col. 12, ll. 33-
36).
[26] The array of target elements is contacted, under hybridization
conditions, with the three labeled nucleic acid populations; the
presence and intensity of each marker is measured at each target
element; and, the ratios of the markers (e.g., first and third markers
and second and third markers) are determined for each target element
(Bao at col. 3, ll. 3-8 and 31-59; col. 6, ll. 22-55).
[27] Comparison of the ratios at a particular target element is said to
provide the copy number for the genomic DNA sequence and for
10
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