Appeal No. 93-3369
Application 07/344,179
70. A diagnostic device for measuring analytes in samples
of biological fluids which comprises:
a column-type assembly defining a fluid pathway having an
open end adapted to receive a sample of biological fluid to be
analyzed, said fluid pathway being bridged by a first solid phase
support, and an effluent discharge point on the side of said
support opposite said open end,
a sleeve-type container having an open end and a closed end,
said column type assembly being received in said open end of said
sleeve-type container,
a specific antibody binder covalently immobilized on said
first solid phase support to which an analyte label is pre-
reacted to saturate substantially all binding sites on said
binder to form a first solid phase specific antibody binder-
analyte label complex, said solid phase complex when contacted
with a biological fluid sample containing a specific analyte,
being adapted to have displaced therefrom labeled analyte in an
amount directly proportional to the concentration of the specific
analyte,
a second solid support, spaced apart from first solid phase
support, housed at the closed end of said sleeve-type container
and in proximity to said effluent discharge point, said second
solid support when contacted by the displaced labeled analyte
from the effluent discharge point of said first solid phase
complex, being adapted to produce a visible color on said second
solid support either directly or after the addition to said
second solid support a substance capable of reacting with the
analyte label to produce a visible color.
The references relied upon by the examiner are:
Graas 4,270,921 June 2, 1981
Liotta 4,446,232 May 1, 1984
Diamond et al. (Diamond) 4,766,062 Aug. 23, 1988
Diekmann 4,956,298 Sep. 11, 1990
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