Appeal No. 93-3369 Application 07/344,179 70. A diagnostic device for measuring analytes in samples of biological fluids which comprises: a column-type assembly defining a fluid pathway having an open end adapted to receive a sample of biological fluid to be analyzed, said fluid pathway being bridged by a first solid phase support, and an effluent discharge point on the side of said support opposite said open end, a sleeve-type container having an open end and a closed end, said column type assembly being received in said open end of said sleeve-type container, a specific antibody binder covalently immobilized on said first solid phase support to which an analyte label is pre- reacted to saturate substantially all binding sites on said binder to form a first solid phase specific antibody binder- analyte label complex, said solid phase complex when contacted with a biological fluid sample containing a specific analyte, being adapted to have displaced therefrom labeled analyte in an amount directly proportional to the concentration of the specific analyte, a second solid support, spaced apart from first solid phase support, housed at the closed end of said sleeve-type container and in proximity to said effluent discharge point, said second solid support when contacted by the displaced labeled analyte from the effluent discharge point of said first solid phase complex, being adapted to produce a visible color on said second solid support either directly or after the addition to said second solid support a substance capable of reacting with the analyte label to produce a visible color. The references relied upon by the examiner are: Graas 4,270,921 June 2, 1981 Liotta 4,446,232 May 1, 1984 Diamond et al. (Diamond) 4,766,062 Aug. 23, 1988 Diekmann 4,956,298 Sep. 11, 1990 2Page: Previous 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 NextLast modified: November 3, 2007