Appeal No. 95-2613
Application 07/876,794
or CG. Boime further describes the expression of said DNA
sequences in Chinese hamster ovary (CHO) cells in order to
obtain heterodimeric hormones. Hellerman describes the
expression of a DNA sequence encoding human parathyroid in rat
pituitary GH cells using a recombinant expression system.
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Clayton describes the construction of vectors encoding a
fusion sequence comprising the DNA sequence encoding the
promoter of the $ subunit of the gonadotropin, luteinizing
hormone (LH) and the DNA sequence encoding the chloramphenical
acetyltransferase (CAT) receptor. The constructs were used to
transform GH cells in order to characterize the activity
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(analyze the inducibility to forskolin) of the LH-$ gene
promoter sequence. Vander discloses that, in humans, FSH, LH,
ACTH, TSH and growth hormone are produced by anterior
pituitary cells. According to the examiner, “[i]t would have
been obvious to a person of ordinary skill in the art to
produce any one of the reproductive hormones of the claims by
using Boime’s method, but instead substituting rat pituitary
cells as the host cells in view of the disclosures of
Hellerman and Clayton that such cells were known in the art to
be used as transfection hosts for the expression of
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