Appeal No. 95-3606
Application 07/827,691
genotype is characterized by an increased amount of
unstable
DNA that maps to the repeat. Most of this unstable DNA
and
indeed most of the repeat in normal X chromosomes is lost
during cloning and DNA amplification by PCR, thus, its
exact
nature must remain speculative.
B. Innis I and Innis II
Innis I teaches that DNA amplification by PCR is useful
to facilitate the cloning of DNA, characterization of both DNA
and RNA sequences, and the detection of pathogens and disease
states associated with the presence of particular DNA nucleic
acid segments (Innis I, col. 1, l. 9-17). “PCR can be used in
conjunction with labeled probes and ‘dot-blot’ methodology to
detect the presence of a nucleic acid sequence initially
present in extraordinarily small amounts” (Innis I, col. 1, l.
44-47). When inefficient or no amplification occurs by PCR,
which unpredictably is often the case, extensive testing is
often required to determine the source of the problem , e.g.,
the primers may be hybridizing to other regions of the target
sequence (Innis I, col. 2, l. 34-50). It is Innis’s intent to
eliminate at least one potential problem, i.e., the formation
and presence of secondary structure in the target DNA molecule
which can greatly reduce the efficiency of amplification in
- 13 -
Page: Previous 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Next
Last modified: November 3, 2007