Appeal No. 1996-3550
Application 08/218,165
Claims 1 and 18 are representative of the subject matter on appeal and read as
follows:
1. A method for providing green note compound, the method comprising the steps of:
a) providing unsaturated fatty acid;
b) providing plant biomass having active levels of lipoxygenase and
hydroperoxide lyase enzymes;
c) providing active alcohol dehydrogenase;
d) providing a mixture by simultaneously contacting the fatty acid, plant
biomass and alcohol dehydrogenase in the presence of an aqueous liquid
under conditions sufficient to:
i) provide release of lipoxygenase and hydroperoxide lyase from the
plant biomass, and
ii) provide reaction of the fatty acid with the lipoxygenase,
hydroperoxide lyase and alcohol dehydrogenase to provide green
note compound;
e) collecting aqueous phase containing green note compound; and
f) separating green note compound from the aqueous phase.
18. A method for providing cis-3-hexen-1-ol, the method comprising the steps of:
a) providing unsaturated fatty acid;
b) providing plant biomass having active levels of lipoxygenase and
hydroperoxide lyase enzymes;
c) providing active alcohol dehydrogenase;
d) providing a mixture by simultaneously contacting the fatty acid, plant
biomass and alcohol dehydrogenase in the presence of an aqueous liquid
under conditions sufficient to:
i) provide release of lipoxygenase and hydroperoxide lyase from the
plant biomass, and
ii) provide reaction of the fatty acid with the lipoxygenase,
hydroperoxide lyase and alcohol dehydrogenase to provide
cis-3-hexen-1-ol;
e) collecting aqueous phase containing cis-3-hexen-1-ol, such aqueous
phase containing greater than 400 ug cis-3-hexen-1-ol per gram of plant
biomass provided in step b); and
f) separating cis-3-hexen-1-ol from the aqueous phase.
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