Ex parte HOLTZ et al. - Page 10




              Appeal No. 1996-3550                                                                                        
              Application 08/218,165                                                                                      
                     Here, appellants apparently concede that the required enzymes and fatty acids are                    
              present in the plant material disclosed in each of the references, but argue that “the fact . . .           
              that various enzymes and [fatty acids] are inherently present in the plant biomass is                       
              irrelevant to the novelty of Appellants’ claims.”  Rather, appellants “submit that the                      
              reference does not contain disclosure of . . . Appellants’ process steps,” and direct                       
              attention to “step (d) of Claim 1, where simultaneous contact of components is clearly set                  
              forth as a process step.”  Brief, pages 10 and 11.                                                          
                     Although not specifically mentioned by the examiner, it is apparent from the                         
              references that endogenous fatty acids, lipoxygenase, hydroperoxide lyase and alcohol                       
              dehydrogenase are released from intracellular compartments upon disruption of the plant                     

              material by crushing or shredding.  For example, Olías teaches that “[t]he formation of C6                  
              aldehydes and alcohols in the plant is related to cell destruction . . . milling of olive fruits is         
              the first step in obtaining the oil” and “[m]illing and malaxation (continuous mixing of                    
              crushed fruit with water) prepare the paste for its extraction . . . [d]isruption of intact cells           
              results in the release of lipid-degrading enzymes that degrade the membrane or stored                       
              lipids.”  Page 2368.  Similarly, Sekiya I teaches that C  volatile compounds are formed                     
                                                                       6                                                  
              “rapidly under mechanical stresses, such as injury or maceration of leaf tissues, during the                
              processing of tea leaf tissues.”  Page 278.                                                                 
                     In our view, the act of crushing or shredding the plant material in an aqueous liquid,               
              disclosed in each of the references, meets the “simultaneously contacting” element of the                   


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