Appeal No. 1996-3550
Application 08/218,165
Claim 1, which represents the invention in its broadest aspect, is directed to a
method comprising providing (a) unsaturated fatty acids, (b) a plant biomass with active
levels of lipoxygenase and hydroperoxide lyase, and (c) active alcohol dehydrogenase;
“simultaneously contacting” (a), (b) and (c) in the presence of an aqueous liquid under
conditions sufficient to produce a green note compound; collecting the aqueous phase
containing a green note compound and separating the green note compound from the
aqueous phase. According to page 13 of the specification, “simultaneously” means that
“within a matter of seconds, the plant material is combined with the fatty acid and yeast in
an aqueous slurry; or the plant material, fatty acid and yeast are combined at essentially
the same time in an aqueous environment.” Claim 2 requires that the alcohol
dehydrogenase is provided in the form of yeast; claim 3 requires that the fatty acid is
linolenic acid, while claim 8 specifies that it includes linoleic acid. Claims 4, 9 and 14
specify that the plant biomass includes watermelon, kale or mustard foliage, respectively;
claim 11 specifies that the biomass contains aldehyde isomerase. Claim 5 requires that
the isolated green note compound includes cis-3-hexen-1-ol. Independent claim 18 is
directed to a method of producing and recovering cis-3-hexen-1-ol at a yield of “greater
than 400 ug cis-3-hexen-1-ol per gram of plant biomass.”
Rejection I
5
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