Interference 102,728
3. Other corroborating evidence relied upon by Singh
a. “Hitzeman SR158:8” and SX 25, Bates No. 483
Singh argues that Dr. Singh discussed his objective with his supervisor and at a
meeting with his coworkers. Paper No. 180, p. 12. Singh relies on “Hitzeman SR158:8”
and SX 25, Bates No. 483 for support. Id. at pp. 12, 17, 19.
Singh’s reliance throughout its brief (Paper No. 180) on “SR158:8,”40 i.e.,
40 We note that in its original brief, Singh relied on the declaration of Dr.
Hitzeman (SR 168, para. 8). Dr. Hitzeman states:
8. On December 14, 1982, I gave a presentation on yeast secretion to
the RRG. In addition to myself, Arjun Singh presented his work on "-factor
yeast secretion. As evidenced by the Research Summary of this meeting
(Document GP530002489-2521) (Singh Exhibit 25, Bates Nos. 000455-000487,
in which all but the pertinent information has been blocked out), Arjun Singh
describes the "-factor gene organization and the structure of the spacer
including the lys arg glu ala glu ala construct at this meeting. Arjun also
indicated at this meeting that his future work would include the removal of these
sequences from the interferon D expression plasmid using site directed
mutagenesis and the construction of expression plasmids with a restriction
following the "-factor preprosequence.
However, even if we assume, arguendo, that Singh intended to rely on Dr.
Hitzeman’s declaration, we find that it fails to corroborate the claim that Dr. Singh had
formulated a definite and permanent idea to employ the new loop deletion mutagenesis
method to remove the nucleotide sequence encoding the glu-ala residues of the "
factor spacer sequence present in the interferon D expression plasmid on December 1,
1982. Dr. Hitzeman not only fails to specify which amino acids would be removed, but
he also fails to mention the technique of loop deletion mutagenesis. To the contrary,
Dr. Hitzeman only mentions site-directed mutagenesis which is further described in
paragraph 9 of his declaration. According to Dr. Hitzeman:
9. During the period 1982 to 1983, site-directed deletion mutagenesis
was a known technique. As set forth in Sambrook, et al. “Molecular Cloning, “
2nd Edition (1989) at pages 15.51 and 15.52 (Singh Exhibit 26, Bates Nos.
000564-566), this technique was known in the early 1970’s and had developed
into an established methodology by 1982.
67
Page: Previous 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 Next
Last modified: November 3, 2007