Interference 103,579
(i) a construct including an a DNA fragment in the
antisense direction which suppresses expression of the GBSS gene
of a potato plant when it is inserted into the potato plant
genome, and
(ii) a method of suppressing amylose formation in
potato tubers, which comprises cultivating a potato plant which
has been genetically engineered to suppress expression of the
GBSS gene by insertion of a construct including a DNA fragment in
the antisense direction which suppresses expression of the GBSS
gene of a potato plant into the potato plant genome.
II. The construct described in Hofvander’s claims for
insertion and/or inserted into the potato plant genome in
antisense direction includes one of three genomic DNA fragments
of the 0-4964 bp PGBSS gene identified as PCT SEQ ID No. 5
(VDX 15/HDX 8).
III. The 0-4964 bp PGBSS gene may be divided into five
segments of ~1000 bp designated as follows (VDX 15/HDX 8):
(i) 0-1000 bp segment,
(ii) 1000-2000 bp segment,
(iii) 2000-3000 bp segment,
(iv) 3000-4000 bp segment, and
(v) 4000-4964 bp segment.
IV. The 0-4964 bp PGBSS gene includes (VDX 15/HDX 8):
(i) a GBSS promoter region including the entire
0-1000 bp segment and a minor fraction of the 1000-2000 bp
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