Interference 103,579
PGBSS gene. We find that the arrow on top of Kuipers’ Fig. 1A,
excluding the dashed line promoter and dashed line terminator
regions, corresponds to the 3.0 kb (3000 b) BamHI-SpeI
sequence identified as SUB10 in Kuipers’ Fig. 1A including
the substantially complete or full length 5'-3' genomic DNA
coding region of the GBSS gene (emphasis added):
For construction pGBA10 and pKGBA10 the 4.2 kb HindIII
fragment containing the complete coding region of the
GBSS gene (Visser et al. 1989) was subcloned in pUC19
(=SUB10; Fig. 1A). The 3.0 kb BamHI-SpeI fragment of
SUB10 was ligated in reversed orientation into digested
pBI121S or pPGB-1S, respectively.
(VDX 4, p. 746, col. 2/HR 337, col. 2);
The full length GBSS cDNA (pGB50, pKGBA50) and genomic
DNA (pGBA10, pKGBA10) constructs were all found to be
capable of complete inhibition of GBSS gene expression
in a higher percentage of transgenic potato clones
(Table 1).
(VDX 4, p. 752, col. 1/HR 343, col. 1); and
For the construction of pGBA10 and pKGBA10 the 3.0 kb
HindIII-SpeI fragment containing the complete coding
region of the GBSS gene . . . was subcloned in pUC19.
(Appendix B, p. 3, l. 6-7).
Kuipers’ 3.0 kb BamHI-SpeI fragment of SUB10 includes the
HindIII-SpeI fragment (VDX 4, p. 748, Fig. 1A, SUB10/HR 339,
Fig. 1A, SUB10):
BamHI HindIII EcoRI NsiI HindIII SstI SpeI
I I I I I I I .
Figure 2 of Hofvander’s involved application instructs that
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