Interference 103,579
insertion in the genome of a potato plant in antisense direction,
is 492 bp in length and includes a minor fraction of the 3000-
4000 bp segment and a minor fraction of the 4000-4964 bp segment
of the 0-4964 bp PGBSS gene depicted in VDX 15 and HDX 8. The 5'
end of the 492 bp fragment (Hofvander’s Antisense fragment III)
comprises a minor fraction of the HindIII-SpeI fragment of the
coding region of the PGBSS gene including the SpeI end of the
HindIII-SpeI fragment (VDX 10 and 2; HR 312 and HDX 8). As best
we can determine, the minor fraction of the HindIII-SpeI fragment
of the coding region of the PGBSS gene including the SpeI end
thereof is the SstI-SpeI subfragment of the HindIII-SpeI fragment
of the coding region of the PGBSS gene. The specification of
Hofvander’s involved application discloses (HR 283, l. 8-14):
The restriction of pSx with SstI and SpeI gives
fragment III (SEQ ID No. 3) which subcloned in pBluescript
. . . is called pBlue3'. Further restriction of pBlue3'
with BamHI-SstI gives a fragment containing 492 bp of
the GBSS gene according to the invention. This fragment
comprises the last intron and exon, translation end and
278 bp of trailer sequence.
XIII. We find that Hofvander’s 342 bp SEQ ID No. 1 and
492 bp SEQ ID No. 3 do not overlap. In the PGBSS gene, these
sequences are separated by a sequence having at least 2000 bp
which includes a major fraction of the 1000-2000 bp segment,
the entire 2000-3000 bp segment, and a major fraction of the
3000-4000 bp segment of the coding region of the PGBSS gene,
which constitute a major fraction of the HindIII-SpeI fragment
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