Interference 103,781
A continuing challenge in controlling pest insects is
the development of insect resistant plants. One approach
to accomplishing this objective is to express proteins
in plants that are toxic to insects. Insect pathogens
such as Bacillus thuringiensis provide a source for
toxin genes. We recently described the cloning and
characterization of full-length and toxic fragments
of the Bacillus thuringiensis HD-73 crystal protein
gene (Adang et. al.)
–More–(38%)
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CONFIDENTIAL MC088384
EXHIBIT 36
2193
Both the complete HD-73 gene endotoxic fragments have
been engineered behind the ma Me (Orf 24) promoter of
pTi15955. These promoter . . . [illegible] cassettes were
cloned into a binary micro T-DNA vector containing a plant
selectable marker and using Agrobacterium tumefaciens
transferred into tobacco plants. We have observed
significant levels of truncated Bt peptides in callus and
immature shoot tissue. However, expanded tobacco leaves
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Last modified: November 3, 2007