Interference 103,781
A. Dr. Murray, Liz Murray, was a post-doc who reported to
me.
Q. And how did you and Dr. Murray come up with the idea
of removing poly-A signals?
A. Well, I had been analyzing - as I said, I was looking
at the - looking at these Bt tobacco plants and looking
at the kill on these plants and seeing that they weren’t
killing like we wanted. And one of the experiments that
Dr. Murray and I did together was a Northern blot experiment
to look at - look at the Bt RNA, because the Bt protein
comes from RNA and we wanted to study the RNA. . . . .
. . . . .
A. The experiment that Dr. Murray did, that I want to talk
a little bit about here, was a Northern blot experiment
to look at the Bt RNA in these plants. And what we found
regarding Bt RNA was that the Bt RNA was - first of all,
there wasn’t very much of it. It was in low amounts.
Okay? And then this RNA was also a type of RNA we call
poly-A RNA. So it had little A’s on the tail. The RNA
was poly-A.
But also importantly, that the RNA was chopped up so
it was truncated. And the truncated size of the RNA was
really - it was too short to make a toxic Bt protein. So
we knew we had this problem.
And then at that time, because we knew - because we
knew that it was poly-A RNA, then we began to think and
study Bt genes for poly-A sites. And we knew that we -
we knew that we needed to remove these poly-A sites that
were stopping transcription so that we could solve this
problem.
Q. Now, is the work that you and Dr. Murray did with the
Northern blot, is that reflected anywhere?
A. Yes. That work is described in Liz Murray’s notebook.
Q. And would you look at Exhibit 492-A? And if we - we
had it retyped since the copy is so poor.
Is this what you are referring to?
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