Interference 103,781
A. Yes, it is.
Q. And what does this page in Dr. Murray’s notebook show?
A. Well, this page in Dr. Murray’s notebook -
. . . . .
The words are written on the page and they’re kind of
retyped up here. And the line I want to point you to, it
says that the Bt band was 1.7 KB [sic, Kb]. That meant
that the Bt, the RNA was shorter than we needed to, so it
was chopped up.
And Liz went on to state, Bt may have some kind of
premature termination signal. And to continue, we spoke
about S-1 mapping the RNA to show where the transcription
was terminated. Then it says, then we could use the
sequenced information to cite specifically mutagenize
the premature termination region of the Bt gene.
What this means is that we saw this chopped-up RNA
and we knew there was a premature termination signal Bt
gene. And this termination signal was causing this
chopped-up RNA.
And then this - the mention of site-specifically
mutagenize the premature termination region of the Bt gene
is that we wanted to go in. We wanted to modify the coding
region of the Bt gene. And because the RNA had a poly-A
tail, we knew that these were poly-A sites in the middle
of the Bt gene that we needed to modify to enhance this
expression.
Q. And what is the date of this notebook page?
A. October 22nd, 1985.
Q. What were you doing in that time frame, late 1985,
towards removing poly-A signals?
A. In - in late 1985, regarding these poly-A signals,
we had - I, personally, was doing a number of computer
searches to identify the number of poly-A sites in Bt
genes and to find out where they’re located so that I
might modify those sites and eliminate them.
-98-
Page: Previous 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 Next
Last modified: November 3, 2007