Interference 103,781
And I replied back to him that I knew a way we could
do that, because I knew that the - the coding usage of the
Bt gene must be different than the typical coding usage of
a plant gene.
I was very familiar with the - what a typical plant
gene looked like. And from looking at the sequence of the
Bt gene, which I had done by that time, I was very aware
that it didn’t look like a plant gene.
. . . . .
Dr. Adang and I thought that seemed like a reasonable
solution to the problem, but he said, well, do we need to
rebuild the whole gene, or is there some section of the gene
that we can focus on to rebuild immediately, to get some
kind of improvement in the sequence.
. . . . .
I pointed out that the very short RNA’s that I had
been seeing, that were about half as long in the Northern
Blots, those might be a clue to what one region we might
want to fix was: That if we could identify the sequences
that were near the end of that particular short RNA, maybe
they would be the sequences which were causing some of the
problems.
I still thought the sequences that were near there
would be fixed by changing them to reflect the codon usage
of the plant gene.
Immediately, thereafter, the questions posed to Dr. Murray
in Delaware I focused on her prior knowledge that “codons with C
and G in the second and third position” and “polyadenylation
signals” in plant genes (AR 4154, p. 455, l. 7, - p. 456,
l. 16):
Q. . . . Doctor, at this time, had you had occasion
to look at the usage of codons with C and G in the second
and third with regard to their usage in plants?
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