Interference 103,781
And one of the modifications that we did to test this
kind of experiment was called a deletion analysis.
Q. What is deletion analysis?
A. In deletion analysis, you take a regular gene and
you make it shorter and shorter and shorter by digesting
away more and more of the gene. And then you take those
different size deletions and you sequence them so you know
exactly how big they are. And then you can do your analysis
with those, to see if the shorter genes act differently than
the longer genes.
Q. And why were you doing those assays?
A. Well, I was doing those assays because the nuclease
protection assay wasn’t immediately revealing where the end
of the short DNA was. And much of the time I didn’t even
have any short RNA to analyze.
So I felt that if I could modify these shorter genes
and make them shorter and shorter, I eventually get to a
point where the gene worked better and then I could start
rebuilding from that end.
Dr. Murray testified that the experimental procedures in the
use of electroporation which she worked to develop finally
yielded reproducible results and were suitable for her to use in
October of 1986 (AR 4157, p. 467, l. 4-8). Accordingly, she
testified (AR 4157, p. 467, l. 9, - p. 468, l. 7)(emphasis
added):
From October of ‘86 until about January or so,
February of 1988, I worked on electroporation of various
Bt genes and worked first to get the technique to work
so we could make RNA out of the cells and then I worked
on doing my analyses, using electroporation.
Q. Did you ever do any work on analyzing the Bt
sequence, other than what you did in the latter part of
1985?
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