Interference 103,781
Dr. Murray’s declaration is a timeline of her efforts from
November 1985, until June 1988.
Nov. 12, 1985 - Feb. 10, 1986 and Aug. 15, 1986 - Jan. 7,
1987: nuclease protection assays on RNA derived from
Bt transformed tobacco plants; attending scientific
meetings; electroporation experiments; generating codon
usage tables (AR 6884, para. 5);
Feb. 25, 1986 - July 3, 1986 and Nov. 6, 1986 - Mar. 9,
1988: originated the concept of electroporation as a
technique to quickly monitor RNA expression levels and
transcript size; several months to optimize
electroporation conditions (AR 6885, para. 6);
May 26, 1987 - Aug. 26, 1987: experiments as needed to
design a probe for nuclease mapping experiments; began
looking for Genbank Sequence Acquisition numbers for
plant genes (AR 6886, para. 7);
May 4, 1987 - Aug. 23, 1987: performed nuclease protection
analyses and electroporation analyses (AR 6886,
para. 8);
Aug. 26-28, 1987: preparation and presentation at meeting
(AR 6886, para. 9);
Aug. 27, 1987 - June 28, 1988: work obtaining gene
sequences for generating and compiling codon usage
tables (AR 6887-6888, para. 11):
This work involved selecting the sequences of
highly expressed plant genes from the Gen Bank database
and from published scientific articles, confirming
their sequences, and inputting the data into the
computer. Since the GenBank sequence was constantly
updated, I continued compiling the codon usage tables
to be as comprehensive as they could be. To be more
efficient, I enlisted the help of Mary Eberle and
several secretaries to update the computer files
while I was performing the experiments to identify
the regions in the Bt gene sequence to modify
first . . . . My notebook pages only reflect
records of when I spent the entire day working on
the codon usage tables.
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