Interference 103,781
A. I was very interested in developing electroporation
so that I could use it to analyze the Bt genes themselves.
. . . .
Dr. Murray considered any time spent developing electroporation
well-spent because (AR 4156, p 464, l. 8-15):
[W]e felt there was a huge advantage to doing this kind of
experiment in electroporation, because if I had to make a
remodeled Bt gene of some kind, and put it into a plant,
that would take three months, four months, possibly even
longer until I had anything to analyze, whereas if I was
able to take the same piece of DNA and put it into a plant
cell, I could get the result out in one hour to two days.
Interestingly, we find from her testimony in Delaware II
that Dr. Murray’s interest in developing electroporation
arose at least in part because of Dr. Murray had doubts that
polyadenylation sequences were causing the shorter RNA sequences
and proposed alternative solutions to the problem (AR 4156, p.
464, l. 25, - AR 4157, p. 466, l. 1)(emphasis added):
Q. Doctor, you said if you made some kind of a
modified DNA. What type of modified DNA did you expect that
you would be able to make based upon this set of experiments
you were doing with the nuclease protection assay?
A. Well, I felt that one way to analyze the nuclease
protection assays was to go in and change the sequences and
remove the polyadenylation sequences.
I also thought the shorter RNA’s might have occurred
due to some other mechanism besides polyadenylation. But
that would have been a completely new mechanism.
And I also thought it might just be the coding region
of the gene was not going to be translated very well by the
plant gene.
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