Interference 103,781
altering the coding sequence of a Bt gene to make that Bt
gene more plant-like by introducing plant preferred codons
is reflected in the statement ‘It appears that for efficient
expression of this toxin in tobacco plants the coding
sequence must be modified to eliminate premature termination
of transcription.’ . . . This statement was removed from the
abstract before it was submitted because this statement
would have revealed our conception regarding our Bt gene
modification method to the public at a time when we were
diligently reducing that conception to practice.
(3) Dr. Adang declared (AR 6878, para. 6):
Following my conception of the invention with Dr.
Murray in November 1985, I sought to determine which
sequence differences were present between Bt and plant
genes, and the regions of the gene which would require
modifications using the methods we had conceived to
increase Bt expression in the transgenic plants. I
performed several computer analyses of Bt genes, including
generating codon usage tables, and determined that the
Bt genes had a strong preference for nucleotides A and T
in the wobble position, and were generally rich in AT
sequences compared to plants . . . .
(4) Dr. Adang further declared (AR 6878-6879, para. 7-9):
(a) “In December of 1985, I also examined Bt genes for
the presence of transcriptional stop sites within the coding
sequence which would be modified using plant preferred
codons . . . [;]”
(b) “From December of 1985 into January of 1986, I
performed sequence comparisons between the cloned Bt genes
to determine if consensus sequences were present in the
coding regions which would need modification . . . [;]” and
(c) From March of 1986 through December of 1987, I
performed sequence analyses of Bt genes to identify RNA
destabilizing sequences which would need to be reduced
and/or eliminated to enable increased Bt gene expression.
In addition, I continued sequence analyses and comparisons
of the Bt genes. Other sequence analyses to locate RNA
destabilizing sequences in Bt genes were performed by
other Agrigenetics scientists. These efforts are reflected
in . . . computer searches . . . .”
-121-
Page: Previous 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 Next
Last modified: November 3, 2007