Interference 103,781
tobacco plants, which had been transformed by Bt DNA
encoding insecticidal protein and expressed low levels of Bt
toxin, reported truncated RNA sequences much shorter than
expected, (AX 101B; AR 4153).
(2) Drs. Adang and Murray suspected that a Bt DNA sequence
encoding Bt toxin could be efficiently expressed in
tobacco plants if it were modified to eliminate the cause
of premature termination of transcription in the plants
(AX 106E).
(3) Drs. Adang and Murray knew that a codon usage table
had been developed by Dr. Adang (AR 0123-0125) and
that a limited amount of codon usage, preference, and
frequency information for plant genes was available
(AR 0094-0097).
(4) Based on the limited amount of codon usage, preference,
and frequency information for Bt and plant genes available,
Drs. Adang and Murray concluded that Bt genes had different
codon usage and different preferred codons than plant genes
(AR 0123-0125).
(5) Drs. Adang and Murray knew that the codon usage,
preference, and frequency data for Bt and plant genes
available for predicting design modifications of Bt DNA
encoding insecticidal protein for efficient expression
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