Barton et al or Fischhoff et al v. Adang et al. - Page 116




          Interference 103,781                                                        
                    A.  I started in November 1985.  I took the shorter               
               RNA’s that we had purified from the transformed tobacco                
               plants and I set about doing a set of experiments to try               
               and identify exactly what sequences were at the end of the             
               short RNA’s.  And those kinds of experiments are called                
               nucleus [sic, nuclease?] protection.                                   
               Dr. Murray proceeded to describe the kind of experiments               
          which were performed and the periods of time over which they were           
          performed.  In example (AR 4156, p. 461, l. 22, - p. 462, l. 4):            
                    Q.  When did you start working on those nuclease                  
               protection assay experiments?                                          
                    A.  I started working on these in November 1985.                  
                    Q.  And how long did they continue?                               
                    A.  Well, I worked on them intermittently, doing other            
               experiments along the way, through August of 1987.  And I              
               still continued to try and complete some experiments after             
               August 1987 until January 1988.                                        
               For other examples, see the following testimony (AR 4156,              
          p. 462, l. 7, - p. 463, l. 3)(emphasis added):                              
                    Q.  What else did you do?                                         
                    A.  Well, I continued to do some Northern experiments,            
               because I was looking for RNA to do the nuclease protection            
               experiments with.  And the nuclease protection experiments             
               were designed to look at the short RNA’s and they weren’t              
               always present in the transformed plants.                              
                    And I continued to work on a new technique that I and             
               another scientist at Agrigenetics were developing so I                 
               could do more rapid analysis of the Bt genes in a plant                
               cell.  And the technique is called electroporation.                    
                                      . . . . .                                       



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