Appeal No. 93-4005
Application 07/795,158
22. With respect to autolysis, Babayan indicates the
following (page 132--emphasis added except for names of
microorganisms; references to publications deleted):
In the process of autolysis the cell wall in
most yeasts undergoes only certain structural
modifications; however, its completeness is
retained, this being observed also at the end of the
process, and the cytoplasmatic material gradually
diffuses into the extra-cellular space. This kind
of autolysis ("endo-type") in yeasts is similar to
that in Bac. subtilis described by Koga and Kusaka,
who observed hollow cells (shadows). In both cases,
autolysis begins with CPM [i.e., cytoplasmatic
membrane,] degradation.
However, autolysis in certain fungi, e.g. in
Schizophyllum commune, is found to involve both CPM and
the cell wall degradation, explained by the highly active
endo- and exo-glucanases in these organisms.
Autolysis depends on the culture age and the
physiological conditions in the cell. Proliferating
culture cells autolyse quicker and to a high degree than
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