Appeal No. 93-4005
Application 07/795,158
10. It is known in the art that it is desirable to
effect lysis of cell walls in fermentation solutions of Ashbya
gossypii to make riboflavin. According to applicant, German
Patent 29 20 592 describes (specification, page 1, line 312
through page 2, line 4):
a process for removing riboflavin from fermenter
suspensions in which the latter are diluted with from 25
to 100% by volume of water and subsequently heated at
from 50 to 65EC for from 15 to 45 minutes in order to
open up the cells containing the riboflavin. The
suspension is cooled and then centrifuged twice to
concentrate riboflavin. In order to increase the
riboflavin content, it is recommended that a proteolytic
enzyme is added to the diluted broth during the heating
or, preferably, thereafter and allowed to act for from 3
to 4 hours.
11. Applicant tells us that there are disadvantages
associated with the process described in the German Patent
German Patent 29 20 592 is similar to Epstein, U.S. Patent Nš 4,165,250 cited by2
the examiner. A translation of the German Patent made by the Patent and Trademark
Office accompanies this opinion. One difference is that the German Patent specifically
mentions Ashbya gossypii, whereas Epstein does not appear to specifically mention Ashbya
gossypii.
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