Appeal No. 93-4005
Application 07/795,158
17. After publication of the application which
matured into the German Patent 29 20 592 and after the filing
date of the application which matured into the Epstein patent,
an article was published describing autolysis in yeasts.
Babayan, "Autolysis in Yeasts," Acta Biotechnol., Vol. 5,
pages 129-136 (1985).
18. According to Babayan, "so-called 'induced
autolysis' of microorganism cells of any age, may be
artificially caused by means of various physical, chemical, or
biological agents" (Babayan, page 130).
19. One physical factor is temperature conditions.
According to Babayan, "[t]he autolysis rate depends on
temperature, and the most optimal temperature interval for
yeast was observed at 45)60EC" (Babayan, page 130, last
sentence).
20. One chemical factor is pH (Babayan, page 131).
21. One biological factor is the "[e]xtent of
aeration. Removal of oxygen from an actively growing culture
of aerobic microorganisms causes impaired energy supply to the
cells" (Babayan, page 131).
- 10 -
Page: Previous 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 Next
Last modified: November 3, 2007