Appeal No. 93-4005
Application 07/795,158
since it increases the viscosity of the broth rather than
decreasing it.
14. There are four examples in the German Patent
29 20 592. None describe the use of the microorganism Ashbya
gossypii. The same is true of the five examples of the
Epstein patent.
15. Epstein, like the German Patent 29 20 592,
states (col. 1, line 54 through col. 2, line 2):
The starting material for the present invention is a
riboflavin fermentation broth. The preparation of
riboflavin from a fermentation broth is known ***.
Briefly, a nutrient medium is sterilized and inoculated
with an organism capable of producing riboflavin. When
the fermentation yield approaches or is at about the
maximum the broth is heated to a temperature of from
about 50EC. to about 65EC. for about from 15 to about 45
minutes, preferably for from about 25 to about 35 minutes
and the riboflavin recovery begins. This heating serves
to lyse the cells and to decrease broth viscosity thus
enhancing the effectiveness of subsequent recovery and
purification steps. Heating beyond about 45 minutes is
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