Appeal No. 93-4005
Application 07/795,158
29 20 592, including large fermenter suspension resulting from
dilution and the cost of proteolytic enzymes (specification,
page 2, lines 8-14).
12. The application which matured into the German
Patent 29 20 592 was first published on December 4, 1980.
13. According to the German Patent 29 20 592 (page
4):
The starting material used in this invention is a
riboflavin fermentation broth. The production of
riboflavin from a fermentation broth is known. In brief,
with this method a nutrient medium is sterilized and
inoculated with a microorganism capable of forming
riboflavin, such as Eremothecium ashbyii or Ashbya
gossypii. When the fermentation yield reaches its
maximum value or approaches this value, then the broth is
heated for 15 to 45 minutes (preferably 25 to 35 minutes)
at 50 to 65EC, after which the riboflavin extraction
begins. The heating causes cell lysis and reduces the
viscosity of the broth, which increases the effectiveness
of the subsequent extraction and purification stages.
Heating for more than 45 minutes is disadvantageous,
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