Appeal No. 93-4005
Application 07/795,158
fermentations (specification, page 1, lines 3-6). In
particular, the invention relates to inducing lysis (i.e.,
breaking down or dissolution) of the cells contained in the
fermentation solution by specific production or activation of
the enzymes which break down the intracellular cell wall and
proteins, i.e., inducing an autolysis (specification, page 2,
lines 25-32).
6. By "autolysis," applicant means "the production
and activation of the intrinsic enzymes which break down the
intracellular cell wall and proteins" (specification, page 2,
lines 33-35).
7. According to applicant, "autolysis of
microorganisms can be induced by stress factors"
(specification, page 3, lines 3-4). Examples of stress
factors are said to include nutrient deficiencies, temperature
increases, oxygen deficiencies, pH changes, and changes in the
pressure in the medium (specification, page 3, lines 5-8).
8. Applicant tells us that riboflavin-producing
strains of Ashbya gossypii can be autolyzed "at from 35 to
55EC within from 6 to 28 hours with almost complete exclusion
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