Appeal No. 93-4005
Application 07/795,158
of oxygen ***. The pH *** should be from 4 to 9"
(specification, page 3, lines 21-26).
9. Claim 6, the only independent claim on appeal,
reads (indentation ours):
A process for increasing the riboflavin content
in spray-dried discharges from riboflavin
fermentations, which comprises
heating a fermentation solution of riboflavin-
producing strains of Ashbya gossypii after
completing (sic--completion) of the riboflavin
fermentation,
at a temperature of from 40 to 50EC and
a pH of from 4 to 9,
for from 6 to 28 hours,
under gentle stirring without aeration,
to induce lysis of cells by specific production
or activation of the intracellular enzymes,
until complete enzymatic breakdown of the cell wall
and proteins occurs.
Prior art
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Last modified: November 3, 2007